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Inducible Operons: lac Operon

Inducible Operons: lac Operon

The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA (thiogalactoside...

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Updated: Jun 25, 2026

Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation

Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation

Published on: November 17, 2017

Inducible Cre mice.

Susanne Feil¹, Nadejda Valtcheva, Robert Feil

¹Interfakultäres Institut für Biochemie, Universität Tübingen, , Tübingen, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|March 7, 2009

Summary

This summary is machine-generated.

The Cre/lox system enables precise control of gene activity in mice. Tamoxifen-inducible CreER recombinases, particularly CreER(T2), facilitate time- and tissue-specific gene knockout studies for disease modeling.

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Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

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Published on: November 21, 2015

Area of Science:

Genetics
Molecular Biology
Developmental Biology

Background:

The Cre/lox site-specific recombination system is crucial for generating conditional somatic mouse mutants.
This system allows for spatial and temporal control of gene activity in mice, aiding gene function studies and disease modeling.

Purpose of the Study:

To describe the mechanism of tamoxifen-dependent CreER recombinases.
To detail the use of CreER recombinases for generating time- and tissue-specific mouse mutants.
To focus on the CreER(T2) recombinase and provide protocols for its application.

Main Methods:

Utilizing ligand-dependent Cre recombinases activated by tamoxifen administration.
Generating transgenic mouse lines expressing CreER(T2).
Inducing recombination via tamoxifen treatment and analyzing outcomes using reporter and target gene studies.

Main Results:

CreER recombinases, especially CreER(T2), offer robust in vivo inducibility.
Established protocols enable the generation of inducible gene knockout mice.
Methods for assessing recombination efficiency and specificity were detailed.

Conclusions:

Tamoxifen-inducible CreER systems, particularly CreER(T2), are powerful tools for conditional gene manipulation in mice.
These systems are valuable for creating sophisticated animal models of human diseases.
The presented protocols serve as general guidelines for Cre/lox-mediated genome modifications in mice.