Related Experiment Video
Updated: Jun 25, 2026
12:04
Mouse Genome Engineering Using Designer Nucleases
Published on: April 2, 2014
Isolation, microinjection and transfer of mouse blastocysts
Susan W Reid1, Lino Tessarollo
1Mouse Cancer Genetics Program, NCI-Frederick, Frederick, MD, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 7, 2009
Summary
This study details generating genetically modified mice. It outlines the process of creating chimeric mice to transfer genetic mutations to the mouse germline for research purposes.
Area of Science:
- Genetics
- Developmental Biology
- Molecular Biology
Background:
- Genetically modified mice are crucial models for studying gene function and disease.
- Homologous recombination in embryonic stem (ES) cells is a primary method for targeted gene modification.
Purpose of the Study:
- To describe the detailed procedures for generating chimeric mice.
- To demonstrate the successful transfer of induced genetic mutations into the mouse germline.
Main Methods:
- Utilizing homologous recombination in embryonic stem (ES) cells to manipulate genes.
- Injecting manipulated ES cells into recipient blastocysts to create chimeric embryos.
- Transferring chimeric blastocysts into foster mice to develop chimeric animals.
Main Results:
- Chimeric mice were successfully generated, with manipulated ES cells populating the germline.
- The induced genetic mutation was transmitted to the offspring of the chimeric mice.
- Homozygous mutant mice were produced through subsequent breeding of heterozygous offspring.
Conclusions:
- The described methods provide a reliable protocol for generating chimeric mice.
- This technique enables the efficient transfer of targeted genetic mutations to the mouse germline.
- These genetically modified mice serve as valuable tools for genetic research and disease modeling.
