Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of Tricaine Methanesulfonate (MS-222) and Alfaxalone Anesthesia in Zebrafish (<i>Danio rerio</i>).

Journal of the American Association for Laboratory Animal Science : JAALAS·2023
Same author

Odorant Binding Causes Cytoskeletal Rearrangement, Leading to Detectable Changes in Endothelial and Epithelial Barrier Function and Micromotion.

Biosensors·2023
Same author

High throughput embryonic zebrafish test with automated dechorionation to evaluate nanomaterial toxicity.

PloS one·2022
Same author

A multistep enrichment process with custom growth medium improves resuscitation of chlorine-stressed coliforms from secondary sewage effluents.

Journal of microbiological methods·2021
Same author

Assessment of zebrafish embryo photomotor response sensitivity and phase-specific patterns following acute- and long-duration exposure to neurotoxic chemicals and chemical weapon precursors.

Journal of applied toxicology : JAT·2020
Same author

Characterization of the continuous skin fibroblastoid cell line, WE-skin11f, from walleye (Sander vitreus).

Journal of fish diseases·2019

Related Experiment Video

Updated: Jun 25, 2026

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity
11:19

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity

Published on: March 7, 2016

Improved cell sensitivity and longevity in a rapid impedance-based toxicity sensor.

Theresa M Curtis1, Joel Tabb, Lori Romeo

  • 1Agave BioSystems, Inc., Ithaca, NY 14850, USA.

Journal of Applied Toxicology : JAT
|March 10, 2009
PubMed
Summary

New cell lines, bovine lung microvessel endothelial cells (BLMVEC) and iguana heart (IgH-2) cells, enhance water toxicity detection. These cells show improved sensitivity and long-term viability for field-portable biosensors.

More Related Videos

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles
04:53

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles

Published on: May 26, 2023

Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer
12:47

Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer

Published on: March 12, 2018

Related Experiment Videos

Last Updated: Jun 25, 2026

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity
11:19

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity

Published on: March 7, 2016

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles
04:53

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles

Published on: May 26, 2023

Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer
12:47

Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer

Published on: March 12, 2018

Area of Science:

  • Environmental Science
  • Cell Biology
  • Biosensor Technology

Background:

  • Developing effective water toxicity sensors is challenging due to the need for both sensitivity and long-term viability.
  • Electric cell-substrate impedance sensing (ECIS) using bovine pulmonary artery endothelial cell (BPAEC) monolayers showed promise but required improvement.

Purpose of the Study:

  • To enhance the toxicant responsiveness and field portability of a cell-based water toxicity sensor.
  • To screen various cell types for improved sensitivity to waterborne industrial chemicals.

Main Methods:

  • Screened human, non-human mammalian, and non-mammalian vertebrate cell lines for sensitivity to 12 industrial chemicals.
  • Assessed toxicant detection using electric cell-substrate impedance sensing (ECIS).
  • Evaluated long-term survival of promising cell lines on ECIS test chips.

Main Results:

  • Bovine lung microvessel endothelial cell (BLMVEC) and iguana heart (IgH-2) cell monolayers detected nine out of 12 chemicals, surpassing the seven detected by BPAEC.
  • Both BLMVEC and IgH-2 cell lines maintained high impedance readings for 37 days on ECIS electrodes.
  • Optimized cell lines demonstrate significant improvement in biosensor performance.

Conclusions:

  • BLMVEC and IgH-2 cells offer enhanced sensitivity and long-term viability for water toxicity detection.
  • These cell lines are crucial for developing a field-portable, cell-based biosensor.
  • Cell line optimization advances the creation of sensitive and robust water quality monitoring tools.