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Related Concept Videos

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
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Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and refractory oxide ion...
Mass Analyzers: Overview01:13

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The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

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Preparing an Isotopically Pure 229Th Ion Beam for Studies of 229mTh
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Improving IRMPD in a quadrupole ion trap.

G Asher Newsome1, Gary L Glish

  • 1Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3290, USA.

Journal of the American Society for Mass Spectrometry
|March 10, 2009
PubMed
Summary
This summary is machine-generated.

Focused lasers enhance infrared multiphoton photodissociation (IRMPD) efficiency in quadrupole ion traps. This method achieves 100% peptide ion dissociation rapidly, even at higher pressures, improving mass spectrometry analysis.

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Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Spectroscopy

Background:

  • Infrared multiphoton photodissociation (IRMPD) is a key technique in mass spectrometry for ion analysis.
  • Achieving efficient dissociation in quadrupole ion traps often requires supplemental ion activation or prolonged irradiation times.
  • Optimizing IRMPD efficiency is crucial for enhancing the speed and sensitivity of mass spectrometry-based analyses.

Purpose of the Study:

  • To investigate the use of a focused laser to improve the efficiency of IRMPD in a quadrupole ion trap.
  • To determine if enhanced laser focusing can achieve complete ion dissociation with shorter irradiation times and without supplemental activation.
  • To explore the impact of bath gas pressure and laser focusing on ion cloud dynamics and dissociation.

Main Methods:

  • Utilized a focused laser beam to deliver increased laser flux onto trapped ion clouds within a quadrupole ion trap.
  • Employed laser tomography to measure the axial amplitudes of ion clouds.
  • Varied bath gas pressure and laser focusing to study their effects on IRMPD efficiency and dissociation times.
  • Analyzed the dissociation of unmodified peptide ions across a range of molecular weights (200 Da to 3 kDa).

Main Results:

  • Achieved up to 100% dissociation of peptide ions using focused laser IRMPD at standard operating pressures (1 x 10(-3) Torr).
  • Demonstrated significantly shorter irradiation times (2.5-25 ms) for complete dissociation compared to unfocused methods.
  • Observed increased sequential dissociation of product ions when the laser was focused and bath gas pressure was optimized.
  • Found that focusing the laser beam to approximate the ion cloud size increased laser flux sixfold.

Conclusions:

  • Focused laser IRMPD offers a highly efficient method for peptide ion dissociation in quadrupole ion traps.
  • This technique enables rapid and complete dissociation without supplemental ion activation, enhancing mass spectrometry workflows.
  • Optimizing laser focusing and bath gas pressure are critical parameters for maximizing dissociation efficiency and minimizing analysis time.