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DNA-binding proteins in mouse cells infected with polyoma virus
Abstract:
DNA-binding proteins from uninfected or polyoma virus-infected mouse cells (3T6) were isolated and compared by DNA-cellulose chromatography and SDS-PAGE. During the early phase of infection, few but significant and reproducible differences were observed. In the 0.4 M NaCl eluate, four major new polypeptides were detected in infected cells, with approximate molecular weights of 160 K, 130 K, 86 K and 81 K, the last two being compatible with previous estimates for polyoma T antigen molecular weight.
Insights
Researchers identified new DNA-binding proteins in polyoma virus-infected mouse cells. Four distinct polypeptides, including potential polyoma T antigen, emerged during early infection, indicating viral influence on cellular proteins.
Area of Science:
- Molecular biology
- Virology
- Cellular biology
Background:
- Polyoma virus infection alters cellular processes.
- Understanding host-pathogen interactions is crucial.
Purpose of the Study:
- To compare DNA-binding proteins in uninfected and polyoma virus-infected mouse cells.
- To identify viral-induced changes in the cellular proteome.
Main Methods:
- Isolation of DNA-binding proteins using DNA-cellulose chromatography.
- Analysis of protein profiles via SDS-PAGE.
- Comparison between infected and uninfected cell extracts.
Main Results:
- Significant differences in DNA-binding proteins were observed during early infection.
- Four new polypeptides (160 K, 130 K, 86 K, 81 K) were detected in infected cells.
- The 86 K and 81 K polypeptides align with known polyoma T antigen molecular weights.
Conclusions:
- Polyoma virus infection induces the expression of specific new DNA-binding proteins.
- These findings contribute to understanding viral oncogenesis and host cell manipulation.
- The identified proteins may play a role in viral replication or transformation.