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Updated: Jun 25, 2026
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Determination of nitrite based on its quenching effect on anodic electrochemiluminescence of CdSe quantum dots
Xuan Liu1, Liang Guo, Lingxiao Cheng
1Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, PR China.
Abstract:
A novel method for electrochemiluminescent (ECL) detection of nitrite was proposed based on its quenching effect on anodic ECL emission of CdSe quantum dots (QDs). The ECL emission could be greatly enhanced by sulfite and dissolved oxygen in a neutral system and occurred at a relatively low potential in comparison with traditional anodic ECL emitter, leading to high sensitivity and good selectivity. The quenching mechanism followed an "electrochemical oxidation inhibition" process, which was completely different from those of some analytes on the ECL emission of QDs. The coincidence of photoluminescence and ECL spectra of the QDs indicated that the ECL emission resulted from the redox process of QDs core and the sulfite acted as a coreactant. The nitrite quenched ECL emission could be analyzed according to the treatment of Stern-Volmer equation with a linear range from 1 microM to 0.5 mM for detection of nitrite. This work presented a new efficient ECL methodology for quencher-related detection.
