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Related Concept Videos

Hydrolysis of ATP01:08

Hydrolysis of ATP

The bonds of adenosine triphosphate (ATP) can be broken through the addition of water, releasing one or two phosphate groups in an exergonic process called hydrolysis. This reaction liberates the energy in the bonds for use in the cell—for instance, to synthesize proteins from amino acids.
If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine monophosphate—by the removal of a second...
ATP Synthase: Structure01:18

ATP Synthase: Structure

ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: Jun 25, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

A sensitive, label free electrochemical aptasensor for ATP detection.

Wang Li1, Zhou Nie, Xiahong Xu

  • 1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.

Talanta
|March 10, 2009
PubMed
Summary

This study developed a sensitive, label-free electrochemical aptasensor using gold nanoparticles (AuNPs) for detecting small molecules like adenosine triphosphate (ATP). The novel AuNPs amplification strategy significantly enhances detection sensitivity and offers a universal platform for molecular sensing.

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Last Updated: Jun 25, 2026

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Area of Science:

  • Electrochemistry
  • Nanotechnology
  • Biosensing

Background:

  • Label-free electrochemical aptasensors are crucial for sensitive small molecule detection.
  • Gold nanoparticles (AuNPs) offer significant signal amplification potential in biosensing applications.

Purpose of the Study:

  • To develop a sensitive, label-free electrochemical aptasensor for small molecular detection.
  • To utilize a gold nanoparticles (AuNPs) amplification strategy for enhanced sensitivity.
  • To demonstrate the feasibility of this aptasensor for adenosine triphosphate (ATP) detection.

Main Methods:

  • Fabrication of a tertiary hybrid DNA-AuNPs system on a gold electrode.
  • Utilizing anchored DNA (ADNA), reporter DNA (RDNA)-tethered AuNPs, and target-responsive DNA (TRDNA).
  • Electrochemical signal generation via chronocoulometric interrogation of [Ru(NH(3))(6)](3+) (RuHex) and monitoring its release upon target binding.

Main Results:

  • The aptasensor demonstrated high sensitivity for adenosine triphosphate (ATP) detection.
  • A wide linear dynamic range of 4 orders of magnitude (1 nM–10 µM) was achieved.
  • A low minimum detectable concentration of 0.2 nM was obtained, showcasing the amplification effect of AuNPs.

Conclusions:

  • The developed AuNPs-based amplification strategy provides a sensitive and label-free method for ATP detection.
  • This approach presents a versatile and universal platform for developing aptasensors for various small molecules.
  • The tertiary hybrid DNA-AuNPs system effectively enhances electrochemical signals for improved molecular detection.