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Updated: Jun 25, 2026
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
An electrochemical biosensor for alpha-fetoprotein based on carbon paste electrode constructed of room temperature
Caifeng Ding1, Fei Zhao, Rui Ren
1The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, PR China.
Abstract:
A novel and effective electrochemical immunosensor for the rapid determination of alpha-fetoprotein (AFP) based on carbon paste electrode (CPE) consisting of room temperature ionic liquid (RTIL) N-butylpyridinium hexafluorophosphate (BPPF(6)) and graphite. The surface of the CPE was modified with gold nanoparticles for the immobilization of the alpha-fetoprotein antibody (anti-AFP). By sandwiching the antigen between anti-AFP on the CPE modified with gold nanoparticles and the secondary antibody, polyclonal anti-human-AFP labeled with horseradish peroxidase (HRP-labeled anti-AFP), the immunoassay was established. The concentration of AFP was determined based on differential pulse voltammetry (DPV) signal, which was generated in the reaction between O-aminophenol (OAP) and H(2)O(2) catalyzed by HRP labeled on the sandwich immunosensor. AFP concentration could be measured in a linear range of 0.50-80.00 ng mL(-1) with a detection limit of 0.25 ng mL(-1). The immunosensor exhibited high sensitivity and good stability, and would be valuable for clinical assay of AFP.
