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Updated: Jun 25, 2026

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS
Published on: August 23, 2024
A combination of molecular probe-tandem electrospray ionization mass spectrometry: a technique for tracing structural
Rumiko Shimizu1, Ai Nagai, Hiroko Tominaga
1Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Tsushimanaka, Okayama, Japan.
Abstract:
An ethyl-labeled phosphatidylcholine hydroperoxide (PC-OOH/Et 2) was synthesized as a molecular probe for naturally occurring PC-OOH 1. Applying the precursor ion scan mode in tandem ESI mass spectrometry at m/z 198, a signal of the PC-OOH/Et 2 alone could be selectively detected even in the presence of a large excess of a complex mixture of phospholipids in the blood. Furthermore, molecular species that formed from PC-OOH/Et 2 by its degradation in the blood were also observed in the same spectrum. Since the molecular probe-and-mass spectrometry-assisted analytical method presented herein requires no separation process by HPLC or TLC and is speedy, requiring less than 1 h, it may be useful in lipid analysis.
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