Related Experiment Video
Updated: Jun 25, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
Published on: August 22, 2019
Search for gross illumination discrepancies in images of natural objects
P George Lovell1, Iain D Gilchrist, David J Tolhurst
1Department of Experimental Psychology, University of Bristol, Clifton, Bristol, UK. p.g.lovell@bris.ac.uk
Abstract:
Shadows may be "discounted" in human visual perception because they do not provide stable, lighting-invariant, information about the properties of objects in the environment. Using visual search, R. A. Rensink and P. Cavanagh (2004) found that search for an upright discrepant shadow was less efficient than for an inverted one. Here we replicate and extend this work using photographs of real objects (pebbles) and their shadows. The orientation of the target shadows was varied between 30 and 180 degrees. Stimuli were presented upright (light from above, the usual situation in the world) or inverted (light from below, unnatural lighting). RTs for upright images were slower for shadows angled at 30 degrees, exactly as found by Rensink and Cavanagh. However, for all other shadow angles tested, the RTs were faster for upright images. This suggests, for small discrepancies in shadow orientation, a switch of processing from a relatively coarse-scaled shadow system to other general-purpose visual routines. Manipulations of the visual heterogeneity of the pebbles that cast the shadows differentially influenced performance. For inverted images, heterogeneity had the expected influence: reducing search efficiency and increasing overall search time. This effect was greatly reduced when images were presented upright, presumably when the distractors were processed as shadows. We suggest that shadows may be processed in a functionally separate, spatially coarse, mechanism. The pattern of results suggests that human vision does not use a shadow-suppressing system in search tasks.
Related Concept Videos
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Difference from Background: Limit of Detection
The LOD indicates the presence or absence...
Super-resolution Fluorescence Microscopy
Light Acquisition

