Related Experiment Video
Updated: Jun 25, 2026
05:27
Stromal Cell Isolation From Hematopoietic Organs
Published on: January 26, 2024
Stromal cells
Muriel Vayssade1, Marie-Danielle Nagel
1CNRS UMR 6600, Technological University of Compiegne, BP 20529 60205 Compiegne Cedex, France.
Frontiers in Bioscience (Landmark Edition)
|March 11, 2009
Summary
Mesenchymal stem cells (MSCs) are versatile cells used in tissue engineering. This review explores biomaterials that support MSCs for regenerating tissues.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Cell Biology
Background:
- Mesenchymal stem cells (MSCs) are multipotent stromal cells.
- MSCs can be isolated from bone marrow, umbilical cord blood, adipose tissue, and amniotic fluid.
- These cells exhibit osteogenic, chondrogenic, and adipogenic differentiation potential.
Purpose of the Study:
- To review biomaterials utilized for tissue engineering with MSCs.
- To highlight the role of biomaterials in guiding MSC differentiation and tissue formation.
Main Methods:
- Literature review of studies on MSCs and biomaterials for tissue engineering.
- Analysis of natural and synthetic biomaterial scaffolds.
- Examination of 3D culture techniques using bioreactors.
Main Results:
- Biomaterial scaffolds (natural and synthetic) support MSC adhesion and differentiation.
- MSCs seeded on scaffolds contribute to new tissue formation.
- Bioreactor-based 3D cultures enhance MSC differentiation for tissue engineering.
Conclusions:
- Biomaterials are crucial for successful tissue engineering with MSCs.
- The choice of biomaterial influences MSC behavior and tissue regeneration outcomes.
- Further research into biomaterial-MSC interactions can advance regenerative medicine.
Related Concept Videos
01:26Stem Cell Niche
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
01:19Multipotency of Hematopoietic Stem Cells
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
01:19Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...
01:01Regulation of Hematopoietic Stem Cells
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
01:33Adult Stem Cells
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...
01:34Production of Formed Elements
Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
Most HSCs commit to...