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Cell cycle changes in transformed cells growing under serum-free conditions
Journal of Cellular Physiology
|March 1, 1977
Summary
Transformed hamster (PyBHK) and mouse (SV3T3) cells grow without serum, but slower. Serum addition increases growth rate proportionally, affecting DNA synthesis rate but not the percentage of cells synthesizing DNA.
Area of Science:
- Cell biology
- Molecular biology
- Cancer research
Background:
- Transformed cell lines like PyBHK and SV3T3 exhibit altered growth properties.
- Serum is a common supplement for cell culture, providing essential growth factors.
Purpose of the Study:
- To investigate the serum-independent growth capabilities of polyoma and SV40 transformed cells.
- To determine the effect of serum concentration on the growth rate and DNA synthesis in these cell lines.
Main Methods:
- Cell culture of PyBHK and SV3T3 cells.
- Serum-free medium cultivation.
- Trypsinization and neutralization for cell transfer.
- Measurement of cell proliferation and DNA synthesis rates.
Main Results:
- PyBHK and SV3T3 cells proliferated in defined medium without serum adaptation.
- Serum-free conditions reduced cell growth rates.
- Increased serum concentration proportionally enhanced growth rates.
- The percentage of DNA-synthesizing cells remained constant, while the rate of DNA synthesis varied with growth rate.
Conclusions:
- Transformed cells can adapt to serum-free conditions, albeit with reduced proliferation.
- Serum concentration directly influences the rate of cell growth and DNA synthesis in transformed cells.
- Cell cycle progression, indicated by DNA synthesis, is modulated by serum availability in transformed cell lines.