Buccal cells submitted to three different storage conditions before DNA extraction

Area of Science:

• Forensic Science
• Molecular Biology
• Genetics

Background:

• DNA extraction from buccal cells (BC) is crucial for various applications.
• Sample storage conditions prior to DNA extraction can affect DNA quality.
• Optimizing pre-analytical steps is essential for reliable genetic analysis.

Purpose of the Study:

• To quantitatively and qualitatively assess the impact of sample storage time before cell lysis solution (CLS) application on DNA extraction from buccal cells.
• To determine the optimal timing for CLS application to preserve DNA integrity.

Main Methods:

• Buccal cells were collected from volunteers and divided into three groups: immediate CLS application, storage at room temperature, and storage at 4°C.
• DNA was extracted using a commercial kit, and subsequently analyzed via spectrophotometry and agarose gel electrophoresis.
• Statistical analysis (one-way ANOVA) was performed on the quantitative DNA yield data.

Main Results:

• No significant quantitative differences in DNA yield were observed across the storage conditions (p=0.385).
• Qualitative analysis revealed DNA degradation in samples stored before CLS application, unlike those with immediate CLS application.
• Immediate CLS application preserved DNA integrity, indicated by the absence of degradation bands on gel electrophoresis.

Conclusions:

• Applying cell lysis solution (CLS) immediately after buccal cell (BC) collection is recommended to obtain high-quality DNA.
• Storage of buccal cell samples prior to CLS application can compromise DNA integrity, despite maintaining quantitative yield.
• Proper pre-analytical handling, including prompt CLS application, is critical for reliable DNA analysis from buccal cells.