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Updated: Jun 25, 2026
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Published on: April 8, 2016
Toponome mapping in prostate cancer: detection of 2000 cell surface protein clusters in a single tissue section and
Walter Schubert1, Anne Gieseler, Andreas Krusche
1Molecular Pattern Recognition Research Group, Medical Faculty, Otto-von-Guericke-University Magdeburg, Germany. walter.schubert@med.ovgu.de
Abstract:
The toponome imaging technology MELC/TIS was applied to analyze prostate cancer tissue. By cyclical imaging procedures, we detected 2100 cell surface protein clusters in a single tissue section. This study provides the whole data set, a new kind of high dimensional data space, solely based on the structure-bound architecture of an in situ protein network, a putative fraction of the tissue code of prostate cancer. It is visualized as a colored mosaic composed of distinct protein clusters, together forming a motif expressed exclusively on the cell surface of neoplastic cells in prostate acini. Cell type specific expression of this motif, found in this preliminary study, suggests that high-throughput toponome analyses of a larger number of cases will provide insight into disease specific protein networks.
