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Updated: Jun 25, 2026

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
Published on: December 30, 2016
Suppressive role of C-terminal binding protein 1 in IL-4 synthesis in human T cells
Noriko Kitamura1, Yuji Motoi, Akio Mori
1Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, Sagamihara, Kanagawa, Japan.
Abstract:
The functional role of C-terminal binding protein (CtBP)1, a transcriptional corepressor, in Th1 and Th2 cytokine expression in human T cells was investigated. Upon introduction of CtBP1 by lentiviral transduction system, IL-4 synthesis was suppressed but IFN-gamma was weakly up-regulated in human CD4(+) T cells. In contrast, a reduction of endogenously expressed CtBP1 in Jurkat T cells using RNAi technology selectively augmented IL-4 expression. The down-regulation of IL-4 by CtBP1 was achieved at the level of gene transcription. Deletion mutation analysis revealed that N-terminal approximately 200 amino acid and C-terminal approximately 50 amino acid residues are participated in CtBP1-mediated suppression of IL-4 expression. CtBP1 expressed in human CD4(+) T cells crucially contribute to Th1/Th2 differentiation via selective down-regulation of IL-4 synthesis.
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