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Updated: Jun 24, 2026

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
Published on: February 7, 2025
High-throughput and high-sensitivity nano-LC/MS and MS/MS for O-glycan profiling
Hasse Karlsson1, Jessica M Holmén Larsson, Kristina A Thomsson
1Department of Medical Biochemistry and Cell Biology, Göteborg University, Gothenburg, Sweden.
Abstract:
Sensitive and fast methods for the profiling of biologically important molecules are highly demanded. Mucins are densely O-glycosylated glycoproteins found at mucosal surfaces and are of great medical interest. Here we describe sensitive methods for the analysis of O-glycans from mucins using gel electrophoresis, and chromatography by nanoLC on graphite columns and structural analysis by electrospray mass spectrometry on a linear trap mass spectrometer.
