Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complementary DNA01:44

Complementary DNA

Overview
Complementary DNA01:44

Complementary DNA

Overview
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decoding the Mechanism of Action of a Parasite TGFβ Antagonist Inspires the Creation of Cell-Type-Specific TGFβ Modulators.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Molecular Engineering of the Helminth TGF-β Mimetics, TGM1 and TGM4, Reveals a Novel Antagonist of TGF-β Signaling in Fibroblasts.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Decoding the Mechanism of Action of a Parasite TGFβ antagonist Inspires the Creation of Cell-type-specific TGFβ Modulators.

bioRxiv : the preprint server for biology·2026
Same author

Argentine Consensus on the Diagnosis of Dementia. Part One: Introduction, Methodology, Current Scenario, and Diagnostic Algorithms

Vertex (Buenos Aires, Argentina)·2026
Same author

Structural and functional analysis of the TGF-β mimic, TGM-2: an immunomodulatory helminth protein.

Genes and immunity·2025
Same author

Key hemodynamic parameters during induced hypothermia cooling phase in healthy and injured piglets.

Respiratory physiology & neurobiology·2025
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2026

A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
12:14

A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters

Published on: June 13, 2019

Generating EST libraries: trans-spliced cDNAs.

Cecilia Fernández1, Rick M Maizels

  • 1Facultad de Química, Universidad de la República, Montevideo, Uruguay.

Methods in Molecular Biology (Clifton, N.J.)
|March 12, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed optimized protocols for creating directional spliced leader cDNA libraries. This method efficiently enriches for specific spliced leader (SL) tagged messenger RNAs (mRNAs) from various organisms.

More Related Videos

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
08:48

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Published on: October 25, 2016

Related Experiment Videos

Last Updated: Jun 24, 2026

A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
12:14

A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters

Published on: June 13, 2019

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
08:48

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Published on: October 25, 2016

Area of Science:

  • Molecular Biology
  • Genomics
  • Parasitology

Background:

  • Eukaryotes utilize trans-splicing for transcript processing, adding a spliced leader (SL) sequence to mRNAs.
  • This SL tagging is characteristic of diverse organisms, including euglenozoa, dinoflagellates, and various metazoan phyla.
  • Previous strategies have used SL tagging to create cDNA libraries for expressed sequence tags (ESTs), particularly from parasitic organisms.

Purpose of the Study:

  • To describe optimized protocols for preparing directional SL-cDNA libraries.
  • To enable the selective cloning of SL-tagged mRNAs from complex RNA mixtures.
  • To facilitate the study of trans-spliced transcriptomes in various biological contexts.

Main Methods:

  • Development of optimized protocols for directional SL-cDNA library preparation.
  • Utilizing PCR amplification of SL-cDNA and subsequent directional cloning into a plasmid vector.
  • Employing small amounts of total RNA as starting material for library construction.

Main Results:

  • The method allows for the selective cloning of specific SL-tagged mRNAs.
  • It effectively enriches for full-length cDNAs from trans-splicing organisms.
  • The protocol is efficient, requiring minimal starting RNA and applicable to diverse samples.

Conclusions:

  • Optimized protocols enable efficient construction of directional SL-cDNA libraries.
  • This technique is valuable for excluding host contamination in parasitic organism studies.
  • The method offers broad applications for characterizing trans-spliced transcriptomes, including in mixed species samples.