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Updated: Jun 24, 2026

3' End Sequencing Library Preparation with A-seq2

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

Expressed sequence tags: an overview.

John Parkinson¹, Mark Blaxter

¹Molecular Structure and Function, Hospital for Sick Children, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|March 12, 2009

Summary

This summary is machine-generated.

Expressed sequence tags (ESTs) are valuable gene discovery tools. This volume details EST generation and analysis, highlighting their expanding applications in phylogenetics and transcript profiling.

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Area of Science:

Molecular Biology
Bioinformatics
Genomics

Background:

Expressed sequence tags (ESTs) are partial mRNA sequences from cDNA libraries.
Over 45 million ESTs from 1400+ eukaryotic species have been generated.
ESTs traditionally aid genome projects and gene discovery.

Purpose of the Study:

To provide practical details on EST generation and analysis.
To explore emerging applications of ESTs.
To serve as a guide for researchers in the field.

Main Methods:

Creation of complementary DNA (cDNA) libraries.
Generation and processing of sequence data.
Bioinformatics analysis of ESTs.

Main Results:

ESTs are increasingly used beyond gene discovery.
Applications now include phylogenetics and transcript profiling.
Proteomics is an emerging application area for ESTs.

Conclusions:

ESTs are versatile tools with growing applications.
Accurate and comprehensive EST data enables new research avenues.
This volume offers a comprehensive guide to EST methodology and utility.