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Related Concept Videos

Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...

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Updated: Jun 24, 2026

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

Riboactivators: transcription activation by noncoding RNA.

Aseem Z Ansari1

  • 1Department of Biochemistry & The Genome Center of Wisconsin, University of Wisconsin-Madison, 53706, USA. ansari@biochem.wisc.edu

Critical Reviews in Biochemistry and Molecular Biology
|March 13, 2009
PubMed
Summary
This summary is machine-generated.

Short RNA duplexes, or noncoding RNAs, are emerging as crucial gene expression regulators. These molecules can activate gene transcription through diverse mechanisms, highlighting their therapeutic potential.

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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
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Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Gene expression is primarily regulated by proteins, but noncoding RNAs (ncRNAs) play significant roles.
  • Historically, ncRNAs were known mainly for gene silencing.
  • Emerging evidence indicates ncRNAs can also activate gene transcription.

Purpose of the Study:

  • To explore the emerging roles of duplex RNA molecules in stimulating gene transcription.
  • To elucidate the diverse mechanisms by which RNA activators up-regulate gene expression.
  • To highlight the growing importance of ncRNAs in gene regulatory networks and cellular health.

Main Methods:

  • Review of recent scientific literature on noncoding RNA function.
  • Analysis of identified mechanisms of RNA-mediated transcriptional activation.
  • Synthesis of findings to illustrate the expanding scope of ncRNA roles.

Main Results:

  • Duplex RNAs function as potent transcriptional activators.
  • Mechanisms include acting as DNA-tethered activation domains, coactivators, and modulators of transcriptional machinery.
  • ncRNAs also regulate other noncoding transcripts, expanding their regulatory influence.

Conclusions:

  • The discovery of RNA activators rapidly expands our understanding of gene regulation.
  • ncRNAs are critical for maintaining cellular homeostasis and are key components of gene regulatory networks.
  • Modulating ncRNA function represents a promising new avenue for therapeutic interventions.