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Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
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Published on: September 6, 2012

Triggered release from peptide-proteinoid microspheres.

Stephen Quirk1

  • 1Kimberly-Clark Corporation, Roswell, Georgia 30076, USA. squirk@kcc.com

Journal of Biomedical Materials Research. Part A
|March 13, 2009
PubMed
Summary
This summary is machine-generated.

Proteinoid microspheres (PMs) can now release drugs on demand. Incorporating specific peptides allows these biocompatible polymers to release contents only when enzymes like matrix metalloproteinases (MMPs) are present.

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Drug Delivery Systems

Background:

  • Proteinoid microspheres (PMs), formed by amino acid condensation, are biocompatible but lack effective triggers for controlled release.
  • Existing PM technology has been limited by difficulties in designing molecules that release encapsulated substances from the PM interior.

Purpose of the Study:

  • To develop a novel trigger mechanism for controlled release from proteinoid microspheres.
  • To investigate the incorporation of peptides into PMs for enzyme-sensitive drug delivery.

Main Methods:

  • Short peptides containing specific enzyme cleavage sites were incorporated into PMs during their synthesis.
  • The resulting hybrid peptide-PMs were tested for their ability to release encapsulated contents in response to specific proteinase activity.
  • Release rates were correlated with the concentration of incorporated peptides.

Main Results:

  • Hybrid peptide-PMs successfully encapsulated small molecules and released them upon enzymatic hydrolysis.
  • PMs containing matrix metalloproteinase (MMP) cleavage site peptides selectively released contents only in the presence of active MMPs.
  • The concentration of incorporated peptides influenced the rate of content release.

Conclusions:

  • Peptide-PMs offer a novel, enzyme-triggered system for controlled release of therapeutic agents.
  • This technology shows potential for targeted drug delivery in conditions requiring fine-tuned proteinase regulation, such as wound healing and periodontal disease.