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Related Experiment Video

Updated: Jun 24, 2026

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates

Published on: April 22, 2019

Collagen type V modulates fibroblast behavior dependent on substrate stiffness.

Roel G M Breuls¹, Darinka D Klumpers, Vincent Everts

¹Department of Physics and Medical Technology, VU University Medical Center, Research Institute MOVE, Van der Boechorststraat 7, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands.

Biochemical and Biophysical Research Communications

|March 13, 2009

Summary

This summary is machine-generated.

Collagen type V impairs fibroblast spreading on soft tissues by altering cell adhesion and actin organization. This suggests collagen V is crucial for cell behavior in developing and healing tissues.

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Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction

Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction

Published on: January 16, 2014

Area of Science:

Biochemistry
Cell Biology
Biomaterials Science

Background:

Collagen type V is upregulated during tissue development and repair.
Its precise role in cell function and extracellular matrix (ECM) organization is not fully understood.
Altered collagen V levels impact ECM structure and tissue stiffness.

Purpose of the Study:

To investigate the combined effects of collagen type V and substrate stiffness on fibroblast behavior.
To analyze changes in fibroblast morphology, focal adhesion formation, and actin organization.

Main Methods:

Fibroblast cultures on substrates coated with collagen I/V or pure collagen I.
Varying substrate stiffness (soft <10 kPa, stiff 68 kPa, glass).
Microscopic analysis of cell morphology, focal adhesions, and actin cytoskeleton.

Main Results:

A collagen I/V coating significantly impaired fibroblast spreading on soft substrates (<10 kPa).
This impairment was not observed on stiffer substrates (68 kPa or glass).
Pure collagen I coating did not affect cell spreading on soft substrates.
Collagen V-induced impairment correlated with diffuse actin staining and smaller focal adhesions.

Conclusions:

Collagen type V plays a critical role in modulating fibroblast behavior, particularly on soft substrates.
The presence of collagen V influences cell spreading, focal adhesion dynamics, and actin organization.
These findings highlight the importance of collagen V in cellular responses during tissue development and remodeling where soft ECM conditions prevail.