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Plasmonic nanoholes in a multichannel microarray format for parallel kinetic assays and differential sensing
Hyungsoon Im1, Antoine Lesuffleur, Nathan C Lindquist
1Laboratory of Nanostructures and Biosensing, Department of Electrical and Computer Engineering, University of Minnesota, Twin Cities, 200 Union Street South East, Minneapolis, Minnesota 55455, USA.
This study introduces a microfluidic chip with nanohole arrays for parallel, real-time molecular binding kinetics measurements. The platform enables label-free detection and high-density arrays for potential protein microarray applications.
Area of Science:
- Nanotechnology
- Biophysics
- Analytical Chemistry
Background:
- Accurate measurement of molecular binding kinetics is crucial for drug discovery and diagnostics.
- Existing methods often require labels or lack high-throughput capabilities.
- Microfluidic devices offer miniaturization and parallel processing advantages.
Purpose of the Study:
- To develop a novel platform for parallel, real-time, and label-free measurement of molecular binding kinetics.
- To integrate nanohole arrays with a microfluidic chip for enhanced biosensing.
- To demonstrate the capability for high-density microarray applications.
Main Methods:
- Fabrication of nanohole arrays in a gold film integrated onto a six-channel microfluidic chip.
- Utilizing surface plasmon resonance (SPR) effects for label-free detection of molecular binding events.
- Employing adjacent negative reference channels to correct for measurement artifacts.
- Measuring streptavidin-biotin binding kinetics at various concentrations.
Main Results:
- Successful real-time, label-free measurement of molecular binding kinetics in parallel across six channels.
- Demonstration of artifact correction using negative reference channels.
- Accurate quantification of streptavidin-biotin binding kinetics.
- Fabrication of a high-density microarray with 252 biosensing pixels (10^6 sensing elements/cm^2).
Conclusions:
- The developed nanohole array-integrated microfluidic chip is a powerful tool for parallel, label-free kinetic analysis.
- The platform demonstrates potential for high-throughput protein microarray applications.
- This technology enables sensitive and accurate molecular interaction studies.
