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Updated: Jun 24, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
Published on: May 5, 2016
Optically modulated fluorophores for selective fluorescence signal recovery
Chris I Richards1, Jung-Cheng Hsiang, Dulal Senapati
1School of Chemistry and Biochemistry and Petit Institute for Biosciences and Bioengineering, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, USA.
Abstract:
Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser, enabling high-sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high-background environments to extract weak signals from completely obscuring background emission.
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