Related Experiment Video
Updated: Jun 24, 2026
Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
Published on: March 5, 2012
Application of retrovirus-mediated expression cloning for receptor screening of a parasite
Kyousuke Kobayashi1, Kentaro Kato, Tatsuki Sugi
1Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
Abstract:
Retrovirus-mediated expression cloning has been applied in both virology and cell biology. Although there is some difficulty in applying this technique to screening for a receptor recognized by an intracellular parasite, we modified the conventional method to identify a putative receptor for the Plasmodium falciparum BAEBL protein. We show that this method is effective in screening for a parasite receptor.
