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Updated: Jun 24, 2026
Purification of a High Molecular Mass Protein in Streptococcus mutans
Published on: September 14, 2019
[Construction and expression of spap/A eukaryotic expression plasmid of Streptococcus mutans in mammalian cells]
Yun-peng Qu1, Jian-guo Liu, Ping Yang
1The Affiliated Stomatological Hospital, Wenzhou Medical College, Wenzhou 325027, China. quyunpeng618@163.com
Purpose:
The study is aimed to evaluate the expression of recombinant plasmid pVAX1-spap/A of surface protein antigen A of Streptococcus mutans in mammalian cells COS-7.
Methods:
The eukaryotic plasmid carrying encoding gene of spap/A of Streptococcus mutans was constructed and the plasmid introduced into COS-7 cells by lipofectamine reagent. The transient expressed protein was detected by immunochemistry technique in COS-7 cells.
Results:
Positive expression was detected in plasma of the cells which were transfected with recombinant plasmid pVAX1-spap/A. The cells which were transfected with pVAX1 were negative.
Conclusions:
Spap/A can translate and express in COS-7 cells after transfected with recombinant plasmid pVAX1-spap/A. The expressed protein locates in the plasma and the protein is able to combine with anti-spap/A antibody. The expressed protein has the antigenicity and recombinant plasmid pVAX1-spap/A is a candidate vaccine.
