Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Bioreactor Controls-I01:28

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Typical Model Studies01:30

Typical Model Studies

Fluid mechanics model studies often utilize scaled-down systems to predict fluid behavior in full-scale environments, such as river flows, dam spillways, and structures interacting with open surfaces. Maintaining Froude number similarity in river models is crucial, as it replicates surface flow features like wave patterns and velocities.
Designing Growth Media for Bioreactors01:30

Designing Growth Media for Bioreactors

Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Water as the often neglected medium at the interface between materials and biology.

Nature communications·2022
Same author

Targeted 2D histology and ultrastructural bone analysis based on 3D microCT anatomical locations.

MethodsX·2021
Same author

Knowledge, consultation time, and choice in breast reconstruction.

The British journal of surgery·2021
Same author

Human and mouse bones physiologically integrate in a humanized mouse model while maintaining species-specific ultrastructure.

Science advances·2020
Same author

Cancer-associated fibroblasts of the prostate promote a compliant and more invasive phenotype in benign prostate epithelial cells.

Materials today. Bio·2020
Same author

Characterisation and evaluation of the regenerative capacity of Stro-4+ enriched bone marrow mesenchymal stromal cells using bovine extracellular matrix hydrogel and a novel biocompatible melt electro-written medical-grade polycaprolactone scaffold.

Biomaterials·2020
Same journal

Valorization of Agricultural Residues Through Nutrient Enrichment for Animal Farming.

Advances in biochemical engineering/biotechnology·2026
Same journal

Safety Aspects of Cell Culture-Derived Food for Human Consumption.

Advances in biochemical engineering/biotechnology·2026
Same journal

Correction to: Perspectives Towards AI and ML.

Advances in biochemical engineering/biotechnology·2026
Same journal

Valorization of Agricultural Residues for Biohydrogen Production via Dark Fermentation.

Advances in biochemical engineering/biotechnology·2026
Same journal

Composting of Agricultural Residues into Organic Fertilizers for Sustainable Agriculture.

Advances in biochemical engineering/biotechnology·2026
Same journal

Correction to: Theoretical Perspectives for Biomolecular Crystallization Prediction.

Advances in biochemical engineering/biotechnology·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2026

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
10:00

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022

Bioreactor studies and computational fluid dynamics.

H Singh1, D W Hutmacher

  • 1Division of Bioengineering, National University of Singapore, Engineering Drive 1, E2-04-01, Singapore, 119260.

Advances in Biochemical Engineering/Biotechnology
|March 18, 2009
PubMed
Summary
This summary is machine-generated.

Computational fluid dynamics (CFD) can help tissue engineers understand bioreactor environments. This technology analyzes fluid forces impacting cell growth and tissue development, moving beyond trial-and-error methods for better tissue engineered constructs.

More Related Videos

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
08:13

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

Published on: December 25, 2015

Power Input Measurements in Stirred Bioreactors at Laboratory Scale
10:49

Power Input Measurements in Stirred Bioreactors at Laboratory Scale

Published on: May 16, 2018

Related Experiment Videos

Last Updated: Jun 24, 2026

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
10:00

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
08:13

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

Published on: December 25, 2015

Power Input Measurements in Stirred Bioreactors at Laboratory Scale
10:49

Power Input Measurements in Stirred Bioreactors at Laboratory Scale

Published on: May 16, 2018

Area of Science:

  • Biomedical Engineering
  • Biotechnology
  • Cell Biology

Background:

  • Bioreactors create hydrodynamic environments influencing tissue engineered constructs (TECs).
  • Current tissue engineering often relies on trial-and-error due to the complex, poorly understood relationship between fluid dynamics and tissue properties.
  • A detailed understanding of fluid mechanics and nutrient transport is crucial for producing clinically useful tissues.

Purpose of the Study:

  • To review the rationale for employing computational fluid dynamics (CFD) in bioreactor studies for tissue engineering.
  • To highlight how CFD can elucidate the impact of hydrodynamic forces on cellular responses within TECs.
  • To advocate for CFD as a critical tool in tissue engineering research.

Main Methods:

  • Review of existing literature on bioreactor hydrodynamics and computational fluid dynamics (CFD).
  • Discussion of the coupling between experimental methods and computational simulations.
  • Analysis of how fluid flow phenomena affect cellular behavior (migration, proliferation, extracellular matrix production).

Main Results:

  • CFD offers a detailed description of fluid mechanics and nutrient transport within bioreactors.
  • The integration of CFD with experimental methods provides synergistic, cohesive datasets.
  • CFD enables visualization and analysis of fluidic forces and stresses on cells and TECs.

Conclusions:

  • CFD is an invaluable tool for tissue engineers to analyze bioreactor environments.
  • Understanding hydrodynamic influences is critical for optimizing TEC development.
  • CFD facilitates a move away from empirical methods towards predictive models in tissue engineering.