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Updated: Jun 24, 2026

Investigating Functional Regeneration in Organotypic Spinal Cord Co-cultures Grown on Multi-electrode Arrays
Published on: September 23, 2015
Premotor nonspiking neurons regulate coupling among motoneurons that innervate overlapping muscle fiber population
Mariano Julián Rodriguez1, Carlos Bernardo Perez-Etchegoyen, Lidia Szczupak
1Dto de Fisiología, Biología Molecular y Celular, FCEN-UBA, IFIBYNE-CONICET, Buenos Aires, Argentina.
Abstract:
Recent work indicated that co-activity of different motoneurons (MNs) in the leech can be regulated through a network that is centered on a pair of nonspiking (NS) neurons. Here, we investigate whether this effect generalizes to different types of MNs that display differential co-activity patterns in different motor behaviors: the dorsal longitudinal excitors DE-3 and the dorsal and ventral excitors MN-L. The data indicates that both motoneurons are coupled to the NS neurons through rectifying junctions that are activated when the motoneuron membrane potential becomes more negative than that of the NS, and that they exert an inhibitory synaptic potential on NS via a polysynaptic pathway. In addition, DE-3 and MN-L are linked by junctions that allow mutual excitation but the transmission of excitatory signals from MN-L to DE-3 depended on NS membrane potential. The results support the view that NS neurons can play a central role in orchestrating the co-activity of MNs during motor behaviors.
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