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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jun 24, 2026

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
06:55

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

Nucleic acid detection and quantification in the developing world.

Jim Huggett1, Clare Green, Alimuddin Zumla

  • 1Centre for Infectious Diseases and International Health, Windeyer Institute for Medical Sciences, University College London, London, UK. j.huggett@ucl.ac.uk

Biochemical Society Transactions
|March 18, 2009
PubMed
Summary
This summary is machine-generated.

Nucleic acid amplification is growing in the developing world, driven by simpler, cheaper methods. This expansion supports local research and diagnostics for infectious diseases and immune responses.

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Last Updated: Jun 24, 2026

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Published on: February 3, 2021

Area of Science:

  • Molecular Biology
  • Global Health
  • Biotechnology

Background:

  • Nucleic acid amplification techniques (NAATs) face challenges in developing regions due to cost and infrastructure limitations.
  • Despite challenges, NAATs are increasingly adopted in countries like Zambia and Tanzania for research and diagnostics.
  • Development of laboratory infrastructure and specialized transport is crucial for scientific research in resource-limited settings.

Purpose of the Study:

  • To highlight the growing impact of nucleic acid amplification in the developing world.
  • To discuss the enabling factors and challenges for implementing NAATs in resource-poor settings.
  • To explore how simpler and cheaper NAATs are facilitating local research and diagnostics.

Main Methods:

  • Review of current trends and applications of nucleic acid amplification in developing countries.
  • Analysis of factors influencing the adoption and impact of NAATs.
  • Examination of emerging methodologies for nucleic acid detection suitable for field use.

Main Results:

  • NAATs are increasingly utilized in research and clinical diagnosis in developing nations.
  • Local scientists are conducting world-leading research using NAATs on infectious diseases and immune responses.
  • Newer, field-appropriate methodologies are being developed to overcome resource limitations.

Conclusions:

  • The impact of nucleic acid amplification in the developing world is expanding.
  • Simpler and more affordable NAATs are key to increasing their role in global health.
  • Continued development of infrastructure and appropriate technologies will further enhance NAATs' contribution to research and diagnosis.