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Slaughtered aged cattle might be one dietary source exhibiting amyloid enhancing factor activity
Takuhiro Yoshida1, Pengyao Zhang, Xiaoying Fu
1Department of Medicine (Neurology and Rheumatology), Shinshu University School of Medicine, Matsumoto, Japan.
Summary
Food products from aged cattle may enhance amyloidosis. Kidney extracts from affected cattle induced severe AA amyloidosis in mice, suggesting potential risks in aged cattle food products.
Area of Science:
- Veterinary Pathology
- Biochemistry
Background:
- Systemic AA amyloidosis is observed in aged cattle.
- Dietary amyloid fibrils can enhance experimental murine AA amyloidosis.
Purpose of the Study:
- To investigate amyloid deposits and amyloid-enhancing activity in aged cattle tissues.
- To assess the potential of aged cattle food products to induce amyloidosis.
Main Methods:
- Histopathological examination of renal and muscular tissues from 293 aged cattle.
- Immunohistochemistry to identify amyloid type (AA).
- SDS-PAGE, Western blot, and mouse injection models to assess amyloid-enhancing activity.
Main Results:
- Renal AA amyloid deposits found in 9/293 cattle; muscular deposits in 1/9.
- Kidney extracts from 4/5 affected cattle showed amyloid-enhancing activity in mice.
- Muscle extracts showed minimal amyloid-enhancing activity.
Conclusions:
- Aged cattle can develop AA amyloid deposits, primarily in the kidney.
- Kidney extracts from affected aged cattle possess significant amyloid-enhancing potential.
- Food products derived from aged cattle may pose a risk for enhancing amyloidosis.
