Related Experiment Video
Updated: Jun 24, 2026

Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas
Published on: February 10, 2018
Functional and biochemical properties of proteins from safflower seed
Ayten Sagiroglu1, Hakki M Ozcan, Aziz Satana
1Department of Chemistry, Section of Biochemistry, Trakya University, Faculty of Science and Letters, Edirne, Turkey.
Abstract:
There are relatively few studies on the properties of proteins that comprise a major part of the safflower seed. The biochemical and functional properties of these proteins have not been fully discovered. In this study, safflower seed proteins were obtained by isoelectric precipitation in two fractions. One of the fractions (Fraction-1) was obtained at pH 10 and the other fraction was obtained, as the protein supernatant separate from the pH precipitate, by ultra filtration (Fraction-2). Functional and biochemical properties of both of fractions were investigated. Gel permeation chromatography (GPC) was applied to both fractions. GPC shows that high-molecular weight constituents are present only in fraction 1, whereas fraction 2 consists of proteins with lower molecular weights in comparison with protein standards. Sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) electrophoresis in the presence of mercaptoethanol and SDS with protein weight markers was applied to both of the fractions. The proteins in both of the fractions were separated and stained with Commassie Brillant blue R-250 dye on the PAGE gel. The molecular weight (M(w)) of each protein band was determined graphically by plotting Log M(w) and relative mobilities (R(f)) using GS-300 scanning densitometry and a suitable computer program.
Related Concept Videos
Protein and Protein Structure
A protein's shape is critical to its function. For example, an enzyme can...
Protein Folding
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Folding
Protein Organization
The primary structure of a protein is its amino acid sequence.
Seed Structure and Early Development of the Sporophyte
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
