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Related Experiment Videos

Quinine uptake by human polymorphonuclear neutrophils.

J el Benna1, C Pasquier, M T Labro

  • 1Laboratoire de la Santé d'Immunologie et d'Hématologie, Centre Hospitalier Universitaire, Institut National de la Santé et de la Recherche Médicale Unité 294, Xavier Bichat, Paris, France.

Antimicrobial Agents and Chemotherapy
|July 1, 1991
PubMed
Summary

Quinine, an antimalarial, is taken up by human polymorphonuclear leukocytes (PMNs) through multiple mechanisms. This uptake, particularly into the soluble fraction, may explain quinine's depressive effects on these immune cells.

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Area of Science:

  • Pharmacology
  • Immunology
  • Cell Biology

Background:

  • The antimalarial drug quinine is known to impair human polymorphonuclear leukocyte (PMN) functions.
  • Understanding the mechanism of quinine's effect on PMNs is crucial for its clinical application.

Purpose of the Study:

  • To investigate the mechanisms of quinine uptake by human PMNs.
  • To quantify quinine accumulation within PMNs and identify cellular compartments involved.

Main Methods:

  • Utilized a fluorometric assay to measure quinine concentration within PMNs.
  • Incubated PMNs with varying concentrations of quinine at 37°C.
  • Performed cell fractionation studies to determine the subcellular localization of quinine.
  • Investigated the influence of temperature, cell viability, and pH on quinine uptake.

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Main Results:

  • PMN-associated quinine reached a cellular-to-extracellular concentration ratio of 140-150 within 5 minutes.
  • Quinine uptake was partially dependent on temperature, cell viability, and extracellular pH.
  • 30-40% of accumulated quinine was found in the particulate fraction, with the remainder in the soluble fraction.
  • Quinine efflux was rapid and complete upon removal of extracellular quinine.

Conclusions:

  • Quinine uptake by PMNs involves multiple mechanisms, including passive association and potentially active transport.
  • A significant portion of quinine accumulates in the soluble fraction of PMNs.
  • These findings provide insights into the cellular mechanisms underlying quinine's depressive effects on PMN function.