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Comparative study of various hydrogen ion buffers to assay Zn(2+)-dependent beta-lactamases
M J Benitez1, M Company, A Arevalillo
1Departamento de Química Fisica Aplicada, Universidad Autónoma de Madrid, Spain.
Antimicrobial Agents and Chemotherapy
|July 1, 1991
Abstract:
The low Zn2+ complex formation constants, the capacity to degrade penicillin G in combination with Zn2+, and UV absorbance make 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 3-[N-tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid, 1,3-bis[tris(hydroxymethyl)methylamino]propane, and 1,4-piperazinediethanesulfonic acid convenient buffers to study Zn(2+)-dependent beta-lactamases.