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Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Published on: March 17, 2023
Regulated renin release from 3T3-L1 adipocytes
Jason D Fowler1, Nathan D Johnson, Thomas A Haroldson
1Department of Integrative Biology, University of Minnesota, Minneapolis, Minnesota 55455, USA.
American Journal of Physiology. Endocrinology and Metabolism
|March 19, 2009
Summary
Adipose tissue renin expression and secretion are regulated during cell differentiation. Forskolin and TNFalpha influence renin mRNA and protein release, with distinct mechanisms observed in 3T3-L1 adipocytes.
Area of Science:
- Biochemistry
- Cell Biology
- Endocrinology
Background:
- Adipose tissue has a local renin-angiotensin system, but renin synthesis and release are not well understood.
- Investigating renin regulation in adipocytes is crucial for understanding local tissue function.
Purpose of the Study:
- To investigate the expression and secretion of renin during 3T3-L1 adipocyte differentiation.
- To determine the effects of forskolin and TNFalpha on renin regulation in mature adipocytes.
Main Methods:
- Utilized differentiating 3T3-L1 cells to analyze renin mRNA and protein activity.
- Treated differentiated adipocytes with forskolin, TNFalpha, IL-6, insulin, and Angiotensin II.
- Assessed the impact of lipase and PKA inhibitors on renin secretion and mRNA levels.
- Compared renin expression in obese mice epididymal adipose tissue to lean controls.
Main Results:
- Renin was undetectable in preadipocytes but upregulated upon differentiation.
- Forskolin increased renin mRNA 28-fold and secretion 12-fold; TNFalpha decreased mRNA fourfold but increased secretion sevenfold.
- Lipase inhibitor (E600) and PKA inhibitor (H89) attenuated forskolin-induced renin release.
- Obese mice showed reduced adipose tissue renin expression, consistent with TNFalpha effects.
Conclusions:
- Renin expression and secretion in adipocytes are complex and influenced by hormonal and metabolic factors.
- Forskolin and TNFalpha differentially regulate renin mRNA and protein secretion via distinct pathways.
- Findings suggest a role for adipose tissue renin in metabolic regulation and obesity.
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