Related Experiment Video
Updated: Jun 24, 2026
Pediatric Animal Model of Extracorporeal Cardiopulmonary Resuscitation After Prolonged Circulatory Arrest
Published on: May 26, 2023
In vitro evaluation of PHISIO-coated sets for pediatric cardiac surgery
Tom N Hoel1, Amrit Singh Thiara, Vibeke Videm
1Department of Thoracic and Cardiovascular Surgery, Rikshospitalet University Hospital, and University of Oslo, Norway. tom.nilsen.hoel@rikshospitalet.no
Objectives:
Phosphorylcholine coated cardiopulmonary bypass (CPB) circuits for children have been available for some years, but conflicting results regarding the inflammatory response have been reported. Accordingly, we aimed to investigate the effect of phosphorylcholine coating on the inflammatory response.
Design:
Ten coated and nine uncoated pediatric CPB sets were tested in an in vitro CPB circuit model. The inflammatory response was assessed by serial assays of hemoglobin, hematocrit, leukocyte counts, platelet counts, activation of the complement system, activation of platelets measured as beta-thromboglobulin, activation of neutrophils measured as myeloperoxidase, activation of coagulation measured as prothrombin fragments 1+2, assessment of hemolysis measured as lactate dehydrogenase, and a panel of seven cytokines. Samples were obtained at baseline and after 15, 30, 60 and 120 minutes.
Results:
There were no significant differences between the phosphorylcholine coated circuits and the uncoated circuits for any of the parameters during the observation period, although a potentially beneficial effect on platelets could not be ruled out.
Conclusions:
Phosphorylcholine coating of CPB equipment did not exert any beneficial effect on the inflammatory markers monitored.
