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Updated: Jun 24, 2026
A Multi-detection Assay for Malaria Transmitting Mosquitoes
Published on: February 28, 2015
Detection of Bacillus sphaericus mosquitocidal toxin genes by multiplex colony PCR
Santosh C Jagtap1, Chandrakant B Jagtap, Pradeep Kumar
1Department of Marine Biotechnology, Ministry of Defence, Anandnagar, Ambernath, Mumbai-421506, India.
Abstract:
A multiplex colony PCR assay was developed for the detection of 5 genes encoding Bacillus sphaericus mosquito larvicidal toxins, namely binA, binB, mtx1, mtx2, and mtx3. Primers designed for these 5 genes yielded specific PCR amplicons of the expected size from type cultures of B. sphaericus. This method of detecting multiple toxin genes by colony PCR in a single tube reaction is a simple, rapid, and economical technique for identification of highly toxic environmental B. sphaericus isolates.