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Related Experiment Video

Updated: Jun 24, 2026

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy
07:40

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy

Published on: August 9, 2019

Immunohistochemical methods for measuring tissue lymphangiogenesis.

Steven Clasper1, David G Jackson

  • 1MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 24, 2009
PubMed
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New marker proteins aid lymphatic research by identifying lymphatic vessels for tumor pathology and isolating cells for basic science. Immunohistochemical analysis of lymphangiogenesis is discussed.

Area of Science:

  • Biomedical research
  • Cell biology
  • Pathology

Background:

  • Lymphatic research has advanced significantly with the discovery of specific marker proteins.
  • These markers enable identification and quantification of lymphatic vessels in tissue samples.
  • They also facilitate the isolation of primary lymphatic endothelial cells for experimental studies.

Purpose of the Study:

  • To focus on the application of lymphatic vessel marker proteins in research.
  • To detail immunohistochemical analysis of lymphangiogenesis using these markers.
  • To discuss protocols and challenges in analyzing lymphatic vessels in tissue sections.

Main Methods:

  • Immunohistochemical analysis of lymphangiogenesis.
  • Utilizing marker proteins for identification and quantitation of lymphatic vessels.

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Related Experiment Videos

Last Updated: Jun 24, 2026

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy
07:40

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy

Published on: August 9, 2019

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections
06:09

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

Published on: February 9, 2020

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
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Published on: March 25, 2020

  • Application on both frozen and paraffin-embedded tissue sections.

    • Main Results:

    • Marker proteins allow precise identification and quantification of lymphatic vessels.
    • Immunohistochemistry enables detailed analysis of lymphangiogenesis.
    • Established protocols are available for analyzing lymphatic vessels in various tissue types.

    Conclusions:

    • Marker proteins are crucial tools in lymphatic research and tumor pathology.
    • Immunohistochemical analysis provides valuable insights into lymphangiogenesis.
    • Further refinement of protocols can address existing challenges in lymphatic vessel research.