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Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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High-throughput Detection Method for Influenza Virus
10:05

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Published on: February 4, 2012

Fluorescent-based techniques for viral detection, quantification, and characterization.

Mathieu Rolland1, Agnès Delaunay, Emmanuel Jacquot

  • 1INRA, Agrocampus Rennes, Le Rheu, France.

Methods in Molecular Biology (Clifton, N.J.)
|March 24, 2009
PubMed
Summary

Fluorescent assays offer sensitive viral detection and characterization. New methods like Single Nucleotide Polymorphism (SNP) and real-time RT-PCR enable detailed viral studies.

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Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • Fluorescent-based technologies provide sensitive, specific, and reliable tools for molecular assays.
  • These tools are crucial for studying viral evolution, epidemiology, and isolate interactions.

Purpose of the Study:

  • To introduce fluorescent-based assays for viral detection, quantification, and characterization.
  • To highlight the simplicity and effectiveness of developing novel molecular assays for viral research.

Main Methods:

  • Utilizing intrinsic characteristics of fluorescent probes for high specificity and sensitivity.
  • Developing Single Nucleotide Polymorphism (SNP) assays for qualitative viral detection.
  • Implementing 'real-time' Reverse Transcription Polymerase Chain Reaction (RT-PCR) for quantitative viral detection.
  • Employing SNaPshot technology for viral isolate characterization.

Main Results:

  • Fluorescent assays enable detailed investigations into viral evolutionary processes, including fitness measurement.
  • Quantitative epidemiology studies can be advanced using these sensitive detection methods.
  • Analysis of synergism and antagonism between closely related viral isolates is facilitated.
  • SNP and real-time RT-PCR assays provide robust qualitative and quantitative viral detection, respectively.
  • SNaPshot technology offers a reliable method for characterizing viral isolates.

Conclusions:

  • Fluorescent-based technologies are versatile and powerful tools for advancing virology research.
  • The development of these assays is straightforward, requiring basic molecular biology techniques.
  • These methods significantly enhance the ability to detect, quantify, and characterize viral agents.