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Updated: Jun 24, 2026

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
Published on: March 22, 2019
SV channels dominate the vacuolar Ca2+ release during intracellular signaling
Igor Pottosin1, Tim Wherrett, Sergey Shabala
1Centro de Investigaciones Biomédicas, Universidad de Colima, Mexico.
Abstract:
Vacuoles have long been suggested to mediate a rise in the cytosolic free Ca(2+) during environmental signal transduction. This study addresses the issue of the control of vacuolar calcium release by some of the known signaling molecules such as IP(3), cADPR, ABA, ATP, cAMP, cGMP, H(2)O(2) and CaM. Over 30 concentrations and/or combinations of these signaling compounds were studied in a series of electrophysiological experiments involving non-invasive ion flux measurements (the MIFE) and patch-clamp techniques. Our results suggest that calcium, calmodulin and nucleotides cause calcium release via SV channels.
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