Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Loss of Major Histocompatibility Complex I and II Function in the Genome of Glacier Lanternfish Provides Insight Into Evolution of Vertebrate Immune Systems.

Genome biology and evolution·2026
Same author

Chromosome-level genome assembly of a Norwegian threespine stickleback (Gasterosteus aculeatus) improves mapping for ancient DNA and QTL associated with parallel loss of pelvic spines.

The Journal of heredity·2026
Same author

Ancient origin and dynamic evolution of bivalent spider toxins.

Molecular biology and evolution·2026
Same author

The origin of the octoploid cloudberry (Rubus chamaemorus) genome is the result of multiple and complex polyploidization events.

The Journal of heredity·2026
Same author

IgM-mediated protection drives early B-cell activation and mucosal containment of <i>Vibrio anguillarum</i> in Atlantic cod (<i>Gadus morhua)</i>.

Frontiers in immunology·2026
Same author

Advancing multiple myeloma therapy: A systematic analysis of corticosteroids and monoclonal antibodies as dual therapeutic agents.

World journal of methodology·2026

Related Experiment Video

Updated: Jun 24, 2026

Cell-Free DNA Integrity Analysis in Urine Samples
07:58

Cell-Free DNA Integrity Analysis in Urine Samples

Published on: January 5, 2017

A solid-phase method for preparing human DNA from urine for diagnostic purposes.

Huma Siddiqui1, Alexander J Nederbragt, Kjetill S Jakobsen

  • 1Department of Biology, Centre for Ecological and Evolutionary Synthesis, University of Oslo, P.O. Box 1066 Blindern, 0316 Oslo, Norway.

Clinical Biochemistry
|March 24, 2009
PubMed
Summary
This summary is machine-generated.

This study presents a simple, automatable method using paramagnetic beads to isolate human DNA from urine. The purified DNA is suitable for sensitive downstream applications like real-time PCR and large-scale diagnostics.

More Related Videos

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
07:56

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Published on: August 6, 2016

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Related Experiment Videos

Last Updated: Jun 24, 2026

Cell-Free DNA Integrity Analysis in Urine Samples
07:58

Cell-Free DNA Integrity Analysis in Urine Samples

Published on: January 5, 2017

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
07:56

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Published on: August 6, 2016

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Clinical Diagnostics

Background:

  • Urine is a valuable source for human DNA isolation.
  • Existing DNA isolation methods can be time-consuming and labor-intensive.
  • Automation is crucial for high-throughput diagnostic and screening studies.

Purpose of the Study:

  • To develop a simple, automatable method for human DNA isolation from small urine volumes using paramagnetic beads.
  • To ensure the purified DNA is suitable for nucleic acid amplification techniques.
  • To establish a reproducible protocol for large-scale screening and diagnostics.

Main Methods:

  • Unspecific capture of urine cells onto magnetic particles.
  • Cell lysis and subsequent DNA binding to the same bead surface.
  • Automation using a liquid handling robot with a magnetic workstation.

Main Results:

  • Isolated DNA served as a template for sensitive real-time PCR and end-point PCR (GAPDH, K-ras, DD3, p53 genes).
  • DNA yields were comparable or higher than silica spin column extraction.
  • The method demonstrated reliable performance when automated.

Conclusions:

  • The paramagnetic bead method yields purified DNA free from inhibitors.
  • The DNA is suitable for sensitive applications including real-time PCR, genotyping, and sequence variant analysis.
  • Magnetic bead-based automation reduces hands-on time, enabling high-throughput, reproducible protocols for diagnostics.