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Updated: Jun 24, 2026

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
Identification of a lipid kinase as a host factor involved in hepatitis C virus RNA replication
Frédéric H Vaillancourt1, Louise Pilote, Mireille Cartier
1Boehringer Ingelheim (Canada) Ltd, 2100 Cunard St., Laval, QC, Canada H7S 2G5. frederic.vaillancourt@boehringer-ingelheim.com
Abstract:
A functional screen of an adenovirus-delivered shRNA library that targets approximately 4500 host genes was performed to identify cellular factors that regulate hepatitis C virus (HCV) sub-genomic RNA replication. Seventy-three hits were further examined by siRNA oligonucleotide-directed knockdown, and silencing of the PI4KA gene was demonstrated to have a significant effect on the replication of a HCV genotype 1b replicon. Using transient siRNA oligonucleotide transfections and stable shRNA knockdown clones in HuH-7 cells, the PI4KA gene was shown to be essential for the replication of all HCV genotypes tested (1a, 1b and 2a) but not required for bovine viral diarrhea virus (BVDV) RNA replication.
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