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Updated: Jun 24, 2026
Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages
Published on: October 30, 2018
[Construction of eukaryotic expression vectors of basic fibroblast growth factor and transfection in rabbit bone
Zhong-Shi Xu1, De-Ming Xiao, Bo-Wen Lin
1Department of Orthopedics, Shenzhen People's Hospital, Shenzhen, China.
Objective:
To investigate approach and possibility of transferring basic fibroblast growth factor (bFGF) gene into rabbit bone marrow stromal cells (BMSCs).
Methods:
The eukaryotic expression vectors harboring bFGF cDNA were constructed and transfected into rabbit BMSCs mediated by liposome. The transcription and expression of bFGF gene in the transfected BMSCs were detected by means of morphological observation, immunohistochemistry, enzyme-linked immunosorbent assay (ELISA) and RT-PCR. The changes in the biological characteristics of the transfected MSCs were also observed.
Results:
Stable overexpression of bFGF protein was detected in the transfected BMSCs, which showed differentiation towards chondrocyte lineage.
Conclusion:
Stable expression of bFGF gene in transfected BMSCs can induce cell differentiation into chondrocyte lineage.
