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Updated: Jun 24, 2026
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
Published on: November 23, 2015
Label-free detection of B. anthracis spores using a surface plasmon resonance biosensor
Dian-Bing Wang1, Li-Jun Bi, Zhi-Ping Zhang
1State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
Abstract:
This study demonstrates the first use of surface plasmon resonance (SPR) technology for the rapid, sensitive and label-free detection of whole B. anthracis spores. The approach involves the use of an SPR biosensor (Biacore 3000), and a monoclonal antibody which was raised against the B. anthracis spore (mAb 8G3). By means of subtractive inhibition assays, whole B. anthracis spores with concentrations as low as 10(4) colony-forming units (CFU) ml(-1) can be detected within 40 min, and other related Bacillus spores, even in high concentrations, can be differentiated from B. anthracis spores.
