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Related Experiment Video

Updated: Jun 24, 2026

Implementation of a Reference Interferometer for Nanodetection
16:11

Implementation of a Reference Interferometer for Nanodetection

Published on: April 26, 2014

A membrane interferometer.

Prasad V Ganesan1, Steven G Boxer

  • 1Department of Chemistry, Stanford University, Stanford, CA 94305-5080, USA.

Proceedings of the National Academy of Sciences of the United States of America
|March 25, 2009
PubMed
Summary
This summary is machine-generated.

Freestanding phospholipid bilayers near a silicon surface were created for precise measurements. This novel architecture allows for studying membrane properties and protein interactions without direct surface contact.

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Last Updated: Jun 24, 2026

Implementation of a Reference Interferometer for Nanodetection
16:11

Implementation of a Reference Interferometer for Nanodetection

Published on: April 26, 2014

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Published on: December 1, 2016

Area of Science:

  • Biophysics
  • Materials Science
  • Nanotechnology

Background:

  • Solid-supported lipid bilayers often face challenges due to surface interactions, limiting their utility for studying membrane proteins.
  • Developing freestanding membrane architectures is crucial for overcoming these limitations and enabling more accurate biophysical studies.

Purpose of the Study:

  • To assemble and characterize freestanding phospholipid bilayers near a silicon wafer surface.
  • To investigate the curvature and ion transport properties of these freestanding bilayers.
  • To assess the potential of this architecture for studying membrane proteins.

Main Methods:

  • Assembly of freestanding phospholipid bilayers spanning micrometer-sized wells in a Si wafer.
  • Utilizing fluorescence-interference techniques for probing bilayers near a Si/SiO(2) interface.
  • Employing osmotic pressure variations to control bilayer curvature.
  • Incorporating the ionophore gramicidin to induce selective cation permeability.

Main Results:

  • Successfully assembled freestanding phospholipid bilayers near a silicon surface.
  • Demonstrated that bilayer curvature can be modulated by osmotic pressure.
  • Showcased selective monovalent cation permeability upon gramicidin incorporation.
  • Verified the potential for high-precision fluorophore position measurements via interferometry.

Conclusions:

  • The freestanding bilayer architecture effectively minimizes surface interactions, offering a promising platform for membrane biophysics.
  • This method allows for precise, non-contact measurements of bilayer properties and protein behavior.
  • The architecture holds potential for advancing studies of membrane proteins and their functions.