Related Experiment Video
Updated: Jun 24, 2026

Preparation of Matcha Fresh Noodles with Stable Color using Embedding Method and Microwave Treatment
Published on: July 26, 2024
Functional properties of whey proteins affected by heat treatment and hydrodynamic high-pressure shearing
1School of Biomedical and Health Sciences, Faculty of Health, Engineering and Science, Victoria University, PO Box 14428, Melbourne, Victoria 8001 Australia.
Abstract:
Two batches of native whey proteins (WP) were subjected to microfluidization or heat denaturation accompanied by microfluidization, followed by spray drying. Powders were assessed for their solubility, heat stability, coagulation time, and emulsifying and foaming properties. Effects of denaturation and shearing were examined by particle size analysis, differential scanning calorimetry, reducing and nonreducing sodium dodecyl sulfate-PAGE, and size exclusion-HPLC. Heat treatment significantly decreased solubility, whereas the number of microfluidization passes markedly improved solubility. The combined effect of heat and pressure significantly increased heat coagulation time. Emulsifying activity index substantially increased upon heat denaturation and was further enhanced by microfluidization. Emulsion stability appeared unaffected by the combined treatment, but the concentration of adsorbed protein on fat droplets was significantly increased. Foaming properties were diminished by heating. Particle size distribution patterns, sodium dodecyl sulfate-PAGE, and size exclusion-HPLC revealed disappearance of major WP and creation of relatively higher, as well as smaller, molecular weight aggregates as a result of the 2 treatments. The use of heat and microfluidization in combination could be used to stabilize WP against heat by producing microparticulated species that have different surface and colloidal properties compared with native WP. These results have implications for the use of WP as an additive in heat-processed foods.
Related Concept Videos
Protein Denaturation
Pasteurization and Food Preservation
Protein Folding
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Folding
Physical Methods for Controlling Microbial Growth: Temperature
Molecular Chaperones and Protein Folding
The...
