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Effect of pioglitazone on sciatic nerve ischemia/reperfusion injury in rats
Reza Rahimian1, Gohar Fakhfouri, Mohammad R Rasouli
1Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Objective:
Evaluation of the effect of pioglitazone on sciatic nerve ischemia/reperfusion (I/R) injury in rat.
Method:
Sixty rats were divided into 10 groups (n = 6). Treatment groups received 15 mg/kg pioglitazone intraperitoneally 1 h before induction of I/R by clamping the right common iliac and femoral arteries for 3 h. After certain time intervals of reperfusion (0 h, 3 h, 1, 4, and 7 days), the function of the hind limb was assessed using behavioral scores based on gait, grasp, paw position, and pinch sensitivity. The sciatic nerve was removed for light microscopy studies and graded for ischemic fiber degeneration (IFD) and edema. Plasma malondialdehyde (MDA) level was measured as an indicator of lipid peroxidation at the end of reperfusion intervals.
Result:
Behavioral scores were improved in the pioglitazone groups just on the 4th and 7th days of reperfusion (p < 0.05). Comparison of the pioglitazone with the control groups showed significant differences in edema at 4 and 7 days. Although IFD decreased in the pioglitazone group at 7 days of reperfusion, it was not statistically significant. In addition, the MDA level was significantly lower in pioglitazone-treated groups.
Conclusion:
Our results show the protective effect of pioglitazone on sciatic nerve I/R injury.