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Published on: March 22, 2020
Gold nanoparticle-enhanced chemiluminescence detection for CE
Shulin Zhao1, Tianxing Niu, Yaru Song
1College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, P. R. China.
Electrophoresis
|March 25, 2009
Summary
Gold nanoparticles (AuNPs) significantly enhance luminol-hydrogen peroxide chemiluminescence (CL) for sensitive capillary electrophoresis (CE) assays. This AuNP-enhanced CE-CL method enables precise detection of epinephrine and norfloxacin in biological samples.
Area of Science:
- Analytical Chemistry
- Nanotechnology
- Biomedical Science
Background:
- Chemiluminescence (CL) detection coupled with capillary electrophoresis (CE) offers high sensitivity for analyzing biomedical compounds.
- Gold nanoparticles (AuNPs) have emerged as promising enhancers for various analytical techniques due to their unique optical and catalytic properties.
Purpose of the Study:
- To develop a highly sensitive capillary electrophoresis-chemiluminescence (CE-CL) assay for the detection of epinephrine and norfloxacin.
- To investigate the role of gold nanoparticles (AuNPs) in enhancing the luminol-H(2)O(2) chemiluminescence reaction for CE analysis.
- To demonstrate the application of the developed method for quantifying analytes in biological samples.
Main Methods:
- Capillary electrophoresis (CE) coupled with luminol-hydrogen peroxide (H(2)O(2)) chemiluminescence (CL) detection.
- Inclusion of gold nanoparticles (AuNPs) in the running buffer to enhance CL signal.
- Fluorescence spectroscopy to study the interaction between luminol and AuNPs.
- Quantification of epinephrine and norfloxacin in human urine samples.
Main Results:
- The addition of AuNPs significantly improved the sensitivity of the CE-CL detection for both epinephrine and norfloxacin.
- Epinephrine inhibited the AuNP-catalyzed CL, while norfloxacin enhanced it.
- Achieved low detection limits: 6.9x10(-9) M for epinephrine and 7.3x10(-9) M for norfloxacin.
- Fluorescence lifetime measurements indicated stabilization of luminol excited states by AuNPs.
- Successful quantification of epinephrine and norfloxacin in human urine samples was demonstrated.
Conclusions:
- Gold nanoparticles effectively enhance luminol-H(2)O(2) chemiluminescence in CE, leading to highly sensitive assays.
- The developed AuNP-enhanced CE-CL method is suitable for the sensitive and selective detection of epinephrine and norfloxacin.
- This approach holds promise for the analysis of biomedical compounds in complex biological matrices.
