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Related Experiment Video

Updated: Jun 24, 2026

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

A nanoliter fluidic platform for large-scale single nucleotide polymorphism genotyping.

Douglas G Roberts¹, Tom B Morrison, Shau Neen Liu-Cordero

¹BioTrove Inc., Woburn, MA 01810, USA.

|March 26, 2009

Summary

This study introduces a nanofluidic SNP genotyping platform using TaqMan assays. The new system offers comparable accuracy and call rates to existing methods, overcoming cost and technical barriers for large-scale genetic studies.

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Last Updated: Jun 24, 2026

Infinium Assay for Large-scale SNP Genotyping Applications

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Published on: November 19, 2013

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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Published on: May 23, 2018

Area of Science:

Genetics
Molecular Biology
Bioengineering

Background:

Single nucleotide polymorphisms (SNPs) are crucial for understanding genetic traits and diseases.
Validating SNP associations in large cohorts is hindered by high costs and technical challenges of current genotyping platforms.

Purpose of the Study:

To implement and evaluate a nanofluidic SNP genotyping platform.
To assess the performance of a high-density microplate system utilizing TaqMan assays for SNP genotyping.

Main Methods:

Implementation of the 5'-exonuclease, biallelic PCR assay (TaqMan) in a nanofluidic microplate.
Performance assessment using 32 TaqMan SNP genotyping assays targeting human polymorphisms.

Main Results:

The nanofluidic platform demonstrated genotyping call rates comparable to conventional microplate systems.
Accuracies achieved with the nanoliter fluidic system were equivalent to larger volume reactions.

Conclusions:

The developed nanoliter fluidic SNP genotyping platform is a viable, cost-effective alternative for large-scale genetic analysis.
This technology can facilitate broader validation of SNP associations, advancing diagnostics and agricultural genetics.