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Dimethylarsinous acid disturbs cytokinesis
Mari Kitamura1, Koichi Kuroda, Ginji Endo
1Department of Food Science and Nutrition, School of Human Environmental Science, Mukogawa Women's University, 6-46 Ikebiraki-cho, Nishinomiya, Japan. mari_k@mukogawa-u.ac.jp
Bulletin of Environmental Contamination and Toxicology
|March 26, 2009
Summary
Dimethylarsinous acid (DMAIII) causes cells to become tetraploid and multinucleated. DMAIII disrupts mitosis and cytokinesis, leading to abnormal cell division and multinuclear cells in V79 cell lines.
Area of Science:
- Cell Biology
- Toxicology
- Genetics
Background:
- Arsenical compounds are known environmental toxins.
- Dimethylarsinous acid (DMAIII) is a metabolite of inorganic arsenic.
Purpose of the Study:
- To investigate the effects of DMAIII on cell division in V79 cells.
- To determine the impact of DMAIII on mitosis and cytokinesis.
Main Methods:
- V79 cells were treated with varying concentrations of DMAIII.
- Cellular morphology and mitotic status were assessed.
- Actin distribution was visualized using rhodamine-phalloidin staining.
Main Results:
- DMAIII induced tetraploidy and multinucleation in a dose-dependent manner.
- Mitotic arrest was observed at 2.5 microM DMAIII, with no mitosis at 5 microM.
- Abnormal actin localization was detected in DMAIII-treated dividing cells.
Conclusions:
- DMAIII interferes with mitotic spindle formation and cytokinesis.
- DMAIII promotes the formation of multinuclear cells.
- These findings highlight DMAIII's potential as a clastogenic agent affecting cell division.
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