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Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
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Membrane traffic: Golgi stumbles over cilia.
1University of Liverpool, Cancer Research Centre, 200 London Road, Liverpool L3 9TA, UK. fabarr@liv.ac.uk
Current Biology : CB
|March 27, 2009
Summary
Golgins are proteins crucial for Golgi structure. Mice lacking TRIP11/GMAP-210 showed normal Golgi but had defects in cilia formation and function, impacting development.
Area of Science:
- Cell Biology
- Developmental Biology
- Molecular Genetics
Background:
- Golgins are Golgi-associated proteins traditionally linked to maintaining Golgi architecture.
- The specific in vivo functions of individual golgins, particularly concerning organelle morphology and cellular processes, remain incompletely understood.
Purpose of the Study:
- To investigate the in vivo role of the golgin TRIP11/GMAP-210 in mammalian development.
- To determine the impact of TRIP11/GMAP-210 deficiency on Golgi structure and function.
- To explore the relationship between TRIP11/GMAP-210, Golgi, and cilia biology.
Main Methods:
- Generation and analysis of mice lacking the TRIP11/GMAP-210 gene.
- Assessment of Golgi apparatus morphology using electron microscopy.
- Evaluation of cilium formation and function in various tissues.
Main Results:
- Mice deficient for TRIP11/GMAP-210 exhibited normal Golgi stack structure.
- These mice displayed significant developmental abnormalities.
- Defects in primary cilium formation and function were observed in TRIP11/GMAP-210 knockout mice.
Conclusions:
- TRIP11/GMAP-210 is essential for proper cilia development and function, despite not being critical for Golgi stack maintenance.
- This finding highlights a novel role for a golgin protein beyond Golgi architecture, linking it to ciliopathies.
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