Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery and Development of One Monomer Molecularly Imprinted Polymers (OMNiMIPs).

Polymers·2025
Same author

Benzylfentanyl as a Surrogate Template for Fentanyl-Selective Imprinted Polymers.

Polymers·2023
Same author

Analytical Perspectives in the Study of Polyvalent Interactions of Free and Surface-Bound Oligonucleotides and Their Implications in Affinity Biosensing.

International journal of molecular sciences·2023
Same author

Improved Enantioselectivity for Atenolol Employing Pivot Based Molecular Imprinting.

Molecules (Basel, Switzerland)·2018
Same author

Editorial.

Journal of molecular recognition : JMR·2018
Same author

Preparation of molecularly imprinted polymeric fibers using a single bifunctional monomer for the solid-phase microextraction of parabens from environmental solid samples.

Journal of separation science·2015

Related Experiment Video

Updated: Jun 24, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

Analyte separation by OMNiMIPs imprinted with multiple templates.

Jason Lejeune1, David A Spivak

  • 1Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.

Biosensors & Bioelectronics
|March 27, 2009
PubMed
Summary

Molecular imprinting with multiple templates (OMNiMIPs) shows reduced imprinting effects at high template loading. However, OMNiMIPs can still achieve effective molecular recognition and separation, even for enantiomers.

More Related Videos

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
10:32

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

Related Experiment Videos

Last Updated: Jun 24, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
10:32

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

Area of Science:

  • Polymer Chemistry
  • Materials Science
  • Analytical Chemistry

Background:

  • Molecular imprinting creates polymers with specific binding sites for target molecules.
  • Multi-analyte imprinting aims to create materials capable of recognizing multiple compounds simultaneously.
  • Understanding the impact of template loading on multi-analyte imprinted polymers is crucial for optimizing their performance.

Purpose of the Study:

  • To investigate the relationship between template loading and the imprinting effect in polymers imprinted with multiple templates (OMNiMIPs).
  • To evaluate the molecular recognition and separation capabilities of OMNiMIPs under varying template loading conditions.
  • To correlate the physical properties of OMNiMIPs with template concentration and molecular structure.

Main Methods:

  • Synthesis of OMNiMIPs using multiple template molecules.
  • Varying the maximum template loading during the imprinting process.
  • Assessing the imprinting effect and enantioselectivity using specific template compounds (e.g., 1,1'-bi-2-naphthol enantiomers).
  • Characterization of polymer physical properties such as porosity and surface area.

Main Results:

  • The imprinting effect in OMNiMIPs is dependent on maximum template loading.
  • Below maximum loading, multi-template polymers exhibit molecular recognition comparable to single-template polymers.
  • Exceeding maximum template loading significantly reduces the imprinting effect and enantioselectivity.
  • OMNiMIP 8, imprinted with four templates, showed a ~60% loss in enantioselectivity for 1,1'-bi-2-naphthol but maintained separation.
  • OMNiMIPs could separate individual templates from mixtures but not all four concurrently.
  • Porosity and surface area correlated with template concentration, irrespective of molecular structure.

Conclusions:

  • Maximum template loading is a critical parameter influencing the performance of multi-analyte molecularly imprinted polymers.
  • While high loading can decrease selectivity, OMNiMIPs retain significant molecular recognition capabilities.
  • OMNiMIPs offer potential for selective separation of individual analytes from complex mixtures.
  • Template concentration, not structure, dictates the physical properties of OMNiMIPs.